Difference between revisions of "Part:BBa K317999"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K317999 short</partinfo>
 
<partinfo>BBa_K317999 short</partinfo>
  
−
Pseudomonas aeruginosa produces rhamnose-containing glycolipid biosurfactants called rhamnolipids. Rhamnolipid is composed of &#946;-D-(&#946;-D-hydroxyalkanoyloxy)alkanoic acids(HAA) derivatized with one- or two-rhamnose sugars.In rhamnolipid biosynthesis, RhlA is required for the formation of the HAA portion of the molecule and RhlB plays a role of the rhamnosyl transferase. When RhlA and RhlB are expressed in E.coli BL21(DE3) strain, rhamno lipid indicating emulsification ability will be produced.
+
Pseudomonas aeruginosa produces rhamnose-containing glycolipid biosurfactants called rhamnolipids. Rhamnolipid is composed of &#946;-D-(&#946;-D-hydroxyalkanoyloxy)alkanoic acids(HAA) derivatized with one- or two-rhamnose sugars.In rhamnolipid biosynthesis, RhlA is required for the formation of the HAA portion of the molecule and RhlB plays a role of the rhamnosyl transferase. When RhlA and RhlB are expressed in E.coli BL21(DE3) strain, rhamno lipid indicating emulsification ability will be produced. This part includes 2 PstI sites.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:15, 27 October 2010

RhlB

Pseudomonas aeruginosa produces rhamnose-containing glycolipid biosurfactants called rhamnolipids. Rhamnolipid is composed of β-D-(β-D-hydroxyalkanoyloxy)alkanoic acids(HAA) derivatized with one- or two-rhamnose sugars.In rhamnolipid biosynthesis, RhlA is required for the formation of the HAA portion of the molecule and RhlB plays a role of the rhamnosyl transferase. When RhlA and RhlB are expressed in E.coli BL21(DE3) strain, rhamno lipid indicating emulsification ability will be produced. This part includes 2 PstI sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 790
    Illegal PstI site found at 904
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 790
    Illegal PstI site found at 904
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1138
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 790
    Illegal PstI site found at 904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 790
    Illegal PstI site found at 904
    Illegal NgoMIV site found at 31
    Illegal NgoMIV site found at 865
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 381