Difference between revisions of "Part:BBa K395702"
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This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.
 
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.
  
−
It is necessary to add either beta-carotene or the synthesis operon.  
+
It is necessary to add either beta-carotene or the synthesis operon.this part applies to ② and ④
  
 
[[Image:tokyotech_Fig. 1.1.8.1 zeaxanthin pellet and plate.jpg|right|460px|Fig. 1.1.8.1 zeaxanthin pellet and plate]]
 
[[Image:tokyotech_Fig. 1.1.8.1 zeaxanthin pellet and plate.jpg|right|460px|Fig. 1.1.8.1 zeaxanthin pellet and plate]]
 
[[Image:tokyotech_table. 1.1.1.2.jpg|left|450px|figure1]]
 
[[Image:tokyotech_table. 1.1.1.2.jpg|left|450px|figure1]]
 +
[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|450px|Fig. 2-1-1-3.TLC.jpg]]
 +
  
 
These parts contain ''crtZ'' (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in ''E. coli'' strain MG1655.  (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
 
These parts contain ''crtZ'' (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in ''E. coli'' strain MG1655.  (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

Revision as of 02:11, 27 October 2010

rbs+crtZ under pBad promoter for double plasmids

This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.

It is necessary to add either beta-carotene or the synthesis operon.this part applies to ② and ④

Fig. 1.1.8.1 zeaxanthin pellet and plate
figure1
Fig. 2-1-1-3.TLC.jpg


These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961