Difference between revisions of "Part:BBa K395701"
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This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. | This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. | ||
− | It is necessary to add either beta-carotene or the synthesis operon. | + | It is necessary to add either beta-carotene or the synthesis operon. This part applies to ③ and ⑤ |
[[Image:tokyotech_Fig. 1.1.8.1 zeaxanthin pellet and plate.jpg|right|460px|Fig. 1.1.8.1 zeaxanthin pellet and plate]] | [[Image:tokyotech_Fig. 1.1.8.1 zeaxanthin pellet and plate.jpg|right|460px|Fig. 1.1.8.1 zeaxanthin pellet and plate]] |
Revision as of 01:58, 27 October 2010
rbs+crtZ under plac promoter for double plasmids
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.
It is necessary to add either beta-carotene or the synthesis operon. This part applies to ③ and ⑤
These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]