Difference between revisions of "Part:BBa K415022"

Revision as of 01:44, 27 October 2010

PluxR/cI-OR : RBS : mCherry : Term : PtetR : RBS : LuxR : Term : Plux/cI-OR : RBS : LuxI

This is a LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification and TetR-regulated LuxR production. This composite is composed of the parts BBa_K415012(Part:BBa_K415006 on low copy number backbone) and Part:BBa_K415019. It can produce luxR, mCherry, and luxI and is thus auto-inducing. The production of luxR is normally constitutive, but can be repressed by TetR and derepressed by aTc. The production of mCherry and luxI are activated by c6-AHL and luxR, and repressed by cI. This is a test part that generates a reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2748
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K415022 short</partinfo>
-This is a LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification and TetR-regulated LuxR production.
+This is a LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification and TetR-regulated LuxR production. This composite is composed of the parts BBa_K415012([[Part:BBa_K415006]] on low copy number backbone) and [[Part:BBa_K415019]]. It can produce luxR, mCherry, and luxI and is thus auto-inducing. The production of luxR is normally constitutive, but can be repressed by TetR and derepressed by aTc.  The production of mCherry and luxI are activated by c6-AHL and luxR, and repressed by cI. This is a test part that generates a reporter.
 <!-- Add more about the biology of this part here