Difference between revisions of "Part:BBa K349117:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
This part has built-in BsaI cutsites surrounding a BBa_P1005 tetracycline antibiotic resistance cassette. NOTE: this part is in a pSB1A3 backbone.
+
This part has built-in BsaI cutsites surrounding a BBa_P1005 tetracycline antibiotic resistance cassette. . Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method. NOTE: this part is in a pSB1A3 backbone.
  
 
===Source===
 
===Source===

Revision as of 01:15, 27 October 2010

tetR-AB (BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1300


Design Notes

This part has built-in BsaI cutsites surrounding a BBa_P1005 tetracycline antibiotic resistance cassette. . Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method. NOTE: this part is in a pSB1A3 backbone.

Source

pSB1A3

References