Difference between revisions of "Part:BBa K349003"
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<partinfo>BBa_K349003 short</partinfo>
 
<partinfo>BBa_K349003 short</partinfo>
  
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Base Plasmid v.2 for the construction of new Bytes-v2.  The RFP cassette can be removed using the BbsI enzyme and a new AB ORF style part may be inserted into the plasmid allowing for creation parts for the BioBytes Assembly System-v2.
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Base Plasmid v.2 for the construction of new BioBytes 2.0.  The RFP cassette can be removed using the BbsI enzyme and a new AB ORF style part may be inserted into the plasmid allowing for creation parts for the BioBytes Assembly Method 2.0.
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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The Base Plasmids v.2 provide a method to create parts for BioByte Assembly System- v.2.  The RFP coding cassette provides a selection method for plasmids that have the new part inserted.  
+
The Base Plasmids v.2 provide a method to create parts for BioByte Assembly Method 2.0.  The RFP coding cassette provides a selection method for plasmids that have the new part inserted.  
  
  

Revision as of 01:06, 27 October 2010

RFP-BA-BbsI (for construction of BA BioBytes 2.0)

Base Plasmid v.2 for the construction of new BioBytes 2.0. The RFP cassette can be removed using the BbsI enzyme and a new AB ORF style part may be inserted into the plasmid allowing for creation parts for the BioBytes Assembly Method 2.0.

Usage and Biology

The Base Plasmids v.2 provide a method to create parts for BioByte Assembly Method 2.0. The RFP coding cassette provides a selection method for plasmids that have the new part inserted.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
  • 1000
    COMPATIBLE WITH RFC[1000]