Difference between revisions of "Part:BBa K349001:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
This part has built-in BsaI cutsites surrounding the J_00450 RFP cassette.
+
This part has built-in BsaI cutsites surrounding the J00450 RFP cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.
  
 
===Source===
 
===Source===

Latest revision as of 01:03, 27 October 2010

RFP-AB-BsaI (for construction of AB BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1086


Design Notes

This part has built-in BsaI cutsites surrounding the J00450 RFP cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.

Source

pSB1C3

References