Difference between revisions of "Part:BBa K404101"
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| − | <partinfo>BBa_K404101 short</partinfo> | + | <partinfo>BBa_K404101 short</partinfo> <br/> |
| + | This BioBrick is a very GC-rich sequence with several palindromic motifs, which form strong hairpin structures. By using the Virus Construction Kit of the Freiburg iGEM team 2010, any gene of interest, which is cloned between this part and the left ITR can be packaged into the AAV2 capsid. These recombinant AAV2 particles can be used for different applications, and were mainly created for therapeutic usage, including cancer treatment with prodrug activating enzymes or imaging approaches. | ||
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| + | Usage and Biology<br/> | ||
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| + | The AAV2 ITRs serve as primers for the host cells’ DNA polymerase which converts the single-stranded virus genome into double-stranded DNA as a part of the viruses replicative cycle. They also play important roles in the virus integration into and rescue from the hosts genome, the formation of concatamers in the host cell nucleus and the encapsidation of the viral genome(K. Berns 1990). Due to these essential functions, the ITR structures cannot be deleted from a viral vector and need to be delivered in cis. | ||
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[[Image:Freiburg10_VectorplasmidBricks 2.png|thumb|center|480px]]<br> | [[Image:Freiburg10_VectorplasmidBricks 2.png|thumb|center|480px]]<br> | ||
Revision as of 00:21, 27 October 2010
[AAV2]-right-ITR
This BioBrick is a very GC-rich sequence with several palindromic motifs, which form strong hairpin structures. By using the Virus Construction Kit of the Freiburg iGEM team 2010, any gene of interest, which is cloned between this part and the left ITR can be packaged into the AAV2 capsid. These recombinant AAV2 particles can be used for different applications, and were mainly created for therapeutic usage, including cancer treatment with prodrug activating enzymes or imaging approaches.
Usage and Biology
The AAV2 ITRs serve as primers for the host cells’ DNA polymerase which converts the single-stranded virus genome into double-stranded DNA as a part of the viruses replicative cycle. They also play important roles in the virus integration into and rescue from the hosts genome, the formation of concatamers in the host cell nucleus and the encapsidation of the viral genome(K. Berns 1990). Due to these essential functions, the ITR structures cannot be deleted from a viral vector and need to be delivered in cis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]