Difference between revisions of "Part:BBa K349114:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This part is a naked Red Fluorescent Protein. It does not include a promoter, an RBS, and start and stop codons. The promoter and terminator are intended to be reconstituted through adjoining linkers of the BioBytes 2.0 system. This part has built-in BsaI cutsites surrounding RFP which can produce A and B overhangs.
+
This part is a naked Red Fluorescent Protein. It does not include a promoter, an RBS, and start and stop codons. The promoter and terminator are intended to be reconstituted through adjoining linkers of the BioBytes 2.0 system. This part has built-in BsaI cutsites surrounding RFP which can produce the A and B overhangs used in the BioBytes 2.0 system.
  
 
===Source===
 
===Source===

Revision as of 00:10, 27 October 2010

nRFP-AB (BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 564
    Illegal AgeI site found at 676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 687


Design Notes

This part is a naked Red Fluorescent Protein. It does not include a promoter, an RBS, and start and stop codons. The promoter and terminator are intended to be reconstituted through adjoining linkers of the BioBytes 2.0 system. This part has built-in BsaI cutsites surrounding RFP which can produce the A and B overhangs used in the BioBytes 2.0 system.

Source

pSB1C3

References