Difference between revisions of "Part:BBa K415010"
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This composite is composed of the parts BBa_K415000 and BBa_S03119. It can produce both luxR and mCherry. The production of luxR is normally constitutive, but can be repressed by TetR and derepressed by aTc. The production of mCherry is activated by c6-AHL and luxR, and repressed by cI. This is a test part that generates a reporter. It also contains an additional R0065 hybrid promoter appended after the LuxR : Term : Term. | This composite is composed of the parts BBa_K415000 and BBa_S03119. It can produce both luxR and mCherry. The production of luxR is normally constitutive, but can be repressed by TetR and derepressed by aTc. The production of mCherry is activated by c6-AHL and luxR, and repressed by cI. This is a test part that generates a reporter. It also contains an additional R0065 hybrid promoter appended after the LuxR : Term : Term. | ||
| − | Please refer to [[Part:BBa_K415006]] for relevant information about this part's fluorescence in response to AHL induction and when co-transformed with Collins' toggle pTSMa BioBricked as [[Part:BBa_K415300]]. | + | Please refer to [[Part:BBa_K415006]], which does not contain the additional R0065 hybrid promoter after the LuxR : Term : Term, for relevant information about this part's fluorescence in response to AHL induction and when co-transformed with Collins' toggle pTSMa BioBricked as [[Part:BBa_K415300]]. |
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Latest revision as of 23:53, 26 October 2010
This is part K415006 with an appended Plux/cI promoter (R0065)
This composite is composed of the parts BBa_K415000 and BBa_S03119. It can produce both luxR and mCherry. The production of luxR is normally constitutive, but can be repressed by TetR and derepressed by aTc. The production of mCherry is activated by c6-AHL and luxR, and repressed by cI. This is a test part that generates a reporter. It also contains an additional R0065 hybrid promoter appended after the LuxR : Term : Term. Please refer to Part:BBa_K415006, which does not contain the additional R0065 hybrid promoter after the LuxR : Term : Term, for relevant information about this part's fluorescence in response to AHL induction and when co-transformed with Collins' toggle pTSMa BioBricked as Part:BBa_K415300.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]