Difference between revisions of "Part:BBa K382002"
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<partinfo>BBa_K382002 short</partinfo> | <partinfo>BBa_K382002 short</partinfo> | ||
− | Vector for transformation of plants. Cloning in E. Coli, transfer to agrobacteria, integrates in the plant genome. Kan resistance in bacteria, confers Basta resistance in plants. Contains the | + | Vector for transformation of plants. Cloning in E. Coli, transfer to agrobacteria, integrates in the plant genome. Kan resistance in bacteria, confers Basta resistance in plants. Contains the pENTCUP2 promoter upstream of MCS for constitutive expression of gene of interest. |
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Revision as of 23:28, 26 October 2010
pORE Expression Series Vector with BioBrick MCS, Basta resistance
Vector for transformation of plants. Cloning in E. Coli, transfer to agrobacteria, integrates in the plant genome. Kan resistance in bacteria, confers Basta resistance in plants. Contains the pENTCUP2 promoter upstream of MCS for constitutive expression of gene of interest.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5326
Illegal XbaI site found at 5299
Illegal SpeI site found at 5378
Illegal PstI site found at 5356 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5326
Illegal NheI site found at 4766
Illegal SpeI site found at 5378
Illegal PstI site found at 5356
Illegal NotI site found at 5341 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5326
Illegal BglII site found at 5212
Illegal BglII site found at 7771
Illegal BamHI site found at 5312
Illegal XhoI site found at 4790 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5326
Illegal XbaI site found at 5299
Illegal SpeI site found at 5378
Illegal PstI site found at 5356 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5326
Illegal XbaI site found at 5299
Illegal SpeI site found at 5378
Illegal PstI site found at 5356
Illegal NgoMIV site found at 625
Illegal NgoMIV site found at 749
Illegal NgoMIV site found at 2181
Illegal NgoMIV site found at 2772
Illegal NgoMIV site found at 7220
Illegal AgeI site found at 341 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 7241