Difference between revisions of "Part:BBa K404007"

(Replacing page with '__NOTOC__ <partinfo>BBa_K404007 short</partinfo> <!-- Add more about the biology of this part here ===Usage and Biology=== <!-- --> <span class='h3bb'>Sequence and Feature...')
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<partinfo>BBa_K404007 short</partinfo>
 
<partinfo>BBa_K404007 short</partinfo>
  
<html>
 
  
 
<br><br><br><br><!---ViralBricks for Loop replacement--->
 
<center><h1>ViralBricks for Loop replacement</h1></center>
 
<div style="width:935px; height:420px; ">
 
<div style="float:left; width:500px; height:auto; text-align:justify;"><p>
 
ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops around the positions 453 and 587. Functional peptides are integrated in the loop tip which will be present on the surface of viral vectros that have been modified with the corresponding ViralBrick.<br><br>
 
<h3>Different ViralBrick versions:</h3>
 
<ul>
 
<li><a href=>453 integration site</a>
 
<li><a href=>587 integration site</a>
 
<li><a href=>587 integration site with HSPG knock-out</a>
 
</ul>
 
<br><h3>Different functional motifs:</h3>
 
<ul>
 
<li><a href=>Biotinylation Acceptor Peptide (BAP)</a>
 
<li><a href=>His-Affinity tag (His)</a>
 
<li><a href=>RGD-Motif for Integrin targeting</a>
 
<li><a href=>Z34C a antibody binding motif from proein A</a>
 
</ul>
 
All these ViralBricks can be used to integrate functional motifs into the viral capsid of the composite part.
 
</p></div>
 
<div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="https://static.igem.org/mediawiki/2010/5/55/Freiburg10_ViralBricks_function.png" width="400"
 
height="auto"/></div></div>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404201" target="_blank"><img src="https://static.igem.org/mediawiki/parts/f/f4/Freiburg10_ViralBrick-logo-453-BAP.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404202" target="_blank"><img src="https://static.igem.org/mediawiki/parts/b/b7/Freiburg10_ViralBrick-logo-453-His.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404215" target="_blank"><img src="https://static.igem.org/mediawiki/parts/c/ce/Freiburg10_ViralBrick-logo-453-empty.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404203" target="_blank"><img src="https://static.igem.org/mediawiki/parts/6/65/Freiburg10_ViralBrick-logo-453-RGD.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404204" target="_blank"><img src="https://static.igem.org/mediawiki/parts/3/37/Freiburg10_ViralBrick-logo-453-Z34C.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404205" target="_blank"><img src="https://static.igem.org/mediawiki/parts/d/de/Freiburg10_ViralBrick-logo-587-BAP.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404216" target="_blank"><img src="https://static.igem.org/mediawiki/parts/8/82/Freiburg10_ViralBrick-logo-587-empty.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404206" target="_blank"><img src="https://static.igem.org/mediawiki/parts/0/0f/Freiburg10_ViralBrick-logo-587-His.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404207" target="_blank"><img src="https://static.igem.org/mediawiki/parts/1/13/Freiburg10_ViralBrick-logo-587-RGD.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404208" target="_blank"><img src="https://static.igem.org/mediawiki/parts/a/ae/Freiburg10_ViralBrick-logo-587-BLA.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404209" target="_blank"><img src="https://static.igem.org/mediawiki/parts/f/f9/Freiburg10_ViralBrick-logo-587KO-BAP.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404210" target="_blank"><img src="https://static.igem.org/mediawiki/parts/0/0d/Freiburg10_ViralBrick-logo-587KO-empty.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404211" target="_blank"><img src="https://static.igem.org/mediawiki/parts/6/6d/Freiburg10_ViralBrick-logo-587KO-His.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404212" target="_blank"><img src="https://static.igem.org/mediawiki/parts/c/c5/Freiburg10_ViralBrick-logo-587KO-RGD.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404213" target="_blank"><img src="https://static.igem.org/mediawiki/parts/8/83/Freiburg10_ViralBrick-logo-587KO-Z34C.png"  width="auto"
 
height="110px"/></a>
 
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K404214" target="_blank"><img src="https://static.igem.org/mediawiki/parts/0/07/Freiburg10_ViralBrick-logo-587KO-Z34C-Spacer.png"  width="auto"
 
height="110px"/></a>
 
 
 
 
 
</html>
 
  
  

Revision as of 23:17, 26 October 2010

pCMV_[AAV2]-VP123



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2396
    Illegal XhoI site found at 698
    Illegal XhoI site found at 884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2922
    Illegal SapI site found at 1833