Difference between revisions of "Part:BBa K325209"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K325209 short</partinfo>
 
<partinfo>BBa_K325209 short</partinfo>
  
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This is the lux operon from V. fischeri expressed under a pBAD promoter.  It emits light when induced by arabinose.  luxCDE create a substrate which the luciferase complex of luxA and luxB uses to emit light at a blue wavelength.
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This is the wild type luciferase from Luciola cruciata with a luciferin regenerating enzyme both under the control of an arabinose induced promoter.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:27, 26 October 2010

Green Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)

This is the wild type luciferase from Luciola cruciata with a luciferin regenerating enzyme both under the control of an arabinose induced promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
    Illegal SpeI site found at 3044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3044
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1550
    Illegal SapI.rc site found at 3334