Difference between revisions of "Part:BBa K299817"

Revision as of 21:20, 26 October 2010

This part is licensed under
Creative BioCommons

control construct - GFP under J23102

Green Fluorescent Protein is a noninvasive fluorescent marker for gene expression, protein localisation and intracellulat protein targeting. Originally from Aequorea victoria.

Authors
Cloned by Joanna Leszczyńska.
Work supervised by Michał Lower.

Construct design
The part consists of J23102 promoter and B0032 RBS ligated to inv gene from Yersinia pestis (horizontal gene transfer form Yersinia pseudotuberculosis). GFP as marker. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (Bba_K299813 and BBa_K299815) To find out more about it's background and design click here.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 708

@@ Line 4: / Line 4: @@
+<p><b>Green Fluorescent Protein</b> is a noninvasive fluorescent marker for gene expression, protein localisation and intracellulat protein targeting. Originally from <i>Aequorea victoria.</i></p>
+<p>Authors
+<br>
+Cloned by Joanna Leszczyńska.<br>
+Work supervised by Michał Lower.</p>
+<p>Construct design
+<br>
+The part consists of J23102 promoter and B0032 RBS ligated to inv gene from Yersinia pestis (horizontal gene transfer form Yersinia pseudotuberculosis). GFP as marker. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (Bba_K299813 and BBa_K299815) To find out more about it's background and design click here.</p>