Difference between revisions of "Part:BBa I719005"
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===Characterization ''in vitro''=== | ===Characterization ''in vitro''=== | ||
| − | This part has been characterized for temperature sensitivity in the [[Chassis/Cell-Free_Systems|Cell-Free Chassis]] by iGEM Imperial 2007. For more detail, please check the testing [http:// | + | This part has been characterized for temperature sensitivity in the [[Chassis/Cell-Free_Systems|Cell-Free Chassis]] by iGEM Imperial 2007. For more detail, please check the testing [http://2007.igem.org/Imperial/Wet_Lab/Protocols/Prot1.9 protocols]and [http://2007.igem.org/Imperial/Wet_Lab/Results/Res1.9 results]. |
[[Image:I719005 invitro.png|thumb|center|500px| ]] | [[Image:I719005 invitro.png|thumb|center|500px| ]] | ||
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<html><hr><h3 style="color:ltblue;"><a href="http://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Team Warsaw 2010's measurement</a></h3></html> | <html><hr><h3 style="color:ltblue;"><a href="http://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Team Warsaw 2010's measurement</a></h3></html> | ||
| − | Absolute promoter strength: 41,8pg RNA/minute/ug substrate DNA | + | Absolute promoter strength: 41,8pg RNA/minute/ug substrate DNA. It equals 8,92 microPoPS |
Revision as of 20:43, 26 October 2010
T7 Promoter
Constitutive promoter derived from the T7 bacteriophage. Allows high expression of proteins only when the T7 polymerase is present. This part is identical to the part BBa_R0085 which currently hasn't been built.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization in vitro
This part has been characterized for temperature sensitivity in the Cell-Free Chassis by iGEM Imperial 2007. For more detail, please check the testing [http://2007.igem.org/Imperial/Wet_Lab/Protocols/Prot1.9 protocols]and [http://2007.igem.org/Imperial/Wet_Lab/Results/Res1.9 results].
| Parameter | Value and Description |
|---|---|
| Optimum temperature | The T7 promoter has a highest output at 25°C, with a one-fold increase in GFP molecules synthesized compared to 37°C. The T7 promoter also has a minimal amount of output at 4°C. |
| Expression Life-span | The rate of GFP synthesis by the T7 promoter reaches a peak at around 30-60 minutes. |
| Peak Expression | The production of GFP decreases to minimal levels after 2 hours and tends towards nil after 4 hours. |