Difference between revisions of "Part:BBa K382004"

Latest revision as of 20:30, 26 October 2010

pORE Gus Reporter Series Vector with BiOBrick MCS (Kan resistance)

Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has a constitutive plant promoter and in-frame Gus reporter. Conforms to Silver BioBrick Assembly Standard 21.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4766
Illegal XbaI site found at 4781
Illegal SpeI site found at 4795
Illegal PstI site found at 4809
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4766
Illegal SpeI site found at 4795
Illegal PstI site found at 4809
Illegal NotI site found at 4772
Illegal NotI site found at 4802
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4766
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 4766
Illegal suffix found at 4795
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 4766
Plasmid lacks a suffix.
Illegal XbaI site found at 4781
Illegal SpeI site found at 4795
Illegal PstI site found at 4809
Illegal NgoMIV site found at 625
Illegal NgoMIV site found at 749
Illegal NgoMIV site found at 2181
Illegal NgoMIV site found at 2772
Illegal NgoMIV site found at 8486
Illegal NgoMIV site found at 9121
Illegal AgeI site found at 341
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 8970
Illegal SapI.rc site found at 9180

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K382004 short</partinfo>
-Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Basta resistance in plants. Has a constitutive plant promoter and in-frame Gus reporter. Conforms to Silver BioBrick Assembly Standard 21.
+Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has a constitutive plant promoter and in-frame Gus reporter. Conforms to Silver BioBrick Assembly Standard 21.