Difference between revisions of "Part:BBa K404224"
Line 5: Line 5:
 
[[Image:Freiburg10_Rep-VP123_P5-TATA-less 453-Z34C.png|thumb|center|480px]]<br>
 
[[Image:Freiburg10_Rep-VP123_P5-TATA-less 453-Z34C.png|thumb|center|480px]]<br>
  
−
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part here-->
 
===Usage and Biology===
 
===Usage and Biology===
 +
<html>
 +
<div style="width:965px; height:400px; ">
 +
<div style="float:left; width:460px; height:auto;  margin: 0px 5px 0px 5px; text-align:justify;">
 +
The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  <a href=http://www.ncbi.nlm.nih.gov/protein/153106>Staphylococcal Protein A</a>. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
 +
 +
</div>
 +
<div style="float:right; width:480px; height:auto; "><img src="https://static.igem.org/mediawiki/parts/9/98/Freiburg10_ViralBrick_motif_Z34C.png" width="460"
 +
height="auto"/></div></div>
 +
</html>
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 20:02, 26 October 2010

[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-Z34C)
The Z34C antibody binding motif, inserted into the 453 loop of [AAV2]-Rep-VP123_p5-TATAless

Freiburg10 Rep-VP123 P5-TATA-less 453-Z34C.png

Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3713
    Illegal XhoI site found at 1913
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4239
    Illegal BsaI site found at 4421
    Illegal BsaI site found at 4458
    Illegal SapI site found at 3048