Difference between revisions of "Part:BBa K364319"
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This protein contains a fungal Zn(2)-Cys(6) binuclear cluster domain.
 
This protein contains a fungal Zn(2)-Cys(6) binuclear cluster domain.
  
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Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts.
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More on GAL4 DNA binding domain: [http://en.wikipedia.org/wiki/Two-hybrid_screening].
  
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Uniprot GAL4 DNA binding domain: [http://www.uniprot.org/uniprot/P04386].
 +
 +
View GAL4 DNA binding domain crystal structure here: [http://www.rcsb.org/pdb/explore/jmol.do?structureId=3COQ&bionumber=1].
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[[Image:GAL4.jpg|800px|thumb|center|3D structure of dimerized Gal4 DNA binding domain]]
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Description of the RXRa DBD crystal structure with reference here:[http://www.cell.com/structure/retrieve/pii/S0969212608001809].
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Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts.
 
[[Image:Team Debrecen pSB1C3-Gal4.jpg|800px|thumb|center|Picture of gel electrophoresis: Gal4 in pSB1C3 results a 441 bp long insert.]]
 
[[Image:Team Debrecen pSB1C3-Gal4.jpg|800px|thumb|center|Picture of gel electrophoresis: Gal4 in pSB1C3 results a 441 bp long insert.]]
  
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[[Image:Gal4.jpg|800px|thumb|center|3D structure of Gal4 DBD]]
 
  
 
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Revision as of 19:48, 26 October 2010

Gal4 DBD

This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. This protein contains a fungal Zn(2)-Cys(6) binuclear cluster domain.

More on GAL4 DNA binding domain: [http://en.wikipedia.org/wiki/Two-hybrid_screening].

Uniprot GAL4 DNA binding domain: [http://www.uniprot.org/uniprot/P04386].

View GAL4 DNA binding domain crystal structure here: [http://www.rcsb.org/pdb/explore/jmol.do?structureId=3COQ&bionumber=1].

3D structure of dimerized Gal4 DNA binding domain

Description of the RXRa DBD crystal structure with reference here:[http://www.cell.com/structure/retrieve/pii/S0969212608001809].

Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts.

Picture of gel electrophoresis: Gal4 in pSB1C3 results a 441 bp long insert.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137