Difference between revisions of "Part:BBa K374009:Experience"

Revision as of 19:43, 26 October 2010

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Applications of BBa_K374009

Biolector experiment

The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it pR promoter. The plasmid was transformed into E.coli (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. From this construct no GFP expression is expected as repression of the pR promoter by the GogR repressor is tight. As a reference the repressor was removed from the construct allowing GFP expression from the pR promoter.

User Reviews

UNIQea4f3b952195d14e-partinfo-00000000-QINU UNIQea4f3b952195d14e-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K374009===
+== Biolector experiment ==
+The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it pR promoter. The plasmid was transformed into ''E.coli'' (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously.
+From this construct no GFP expression is expected as repression of the pR promoter by the GogR repressor is tight. As a reference the repressor was removed from the construct allowing GFP expression from the pR promoter.
 ===User Reviews===