Difference between revisions of "Part:BBa K404208"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place. | ||
| + | Assembled capsids can easily be detected by beta-lactamase assay. | ||
===Restriction sites=== | ===Restriction sites=== | ||
Revision as of 18:09, 26 October 2010
ViralBrick-587-Beta Lactamase (BLA)
Usage and Biology
ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place.
Assembled capsids can easily be detected by beta-lactamase assay.
===Restriction sites===