Difference between revisions of "Part:BBa K325219"
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THIS PAGE IS CURRENTLY BEING UPDATED.  
 
THIS PAGE IS CURRENTLY BEING UPDATED.  
  
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==Pictures==
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[[Image:300px-Cambridge-Wed.jpg|thumb|569px|center|'''Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with [https://parts.igem.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [https://parts.igem.org/Part:BBa_K325219 BBa 325219] (red light bulb) ''']]
  
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'''Performance'''<br>
 
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<center>
 
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{|{{Table}}
 
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!Experiment<sup>1</sup>
 
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!Characteristic<sup>1</sup>
 
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!Value<sup>1</sup>
 
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|-
 
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|rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]
 
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|''Maximum Output''
 
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|6.6 [[PoPS]] cell<sup>-1</sup>
 
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|-
 
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|''Hill coefficient''
 
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|1.6
 
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|-
 
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|[[Switch Point|''Switch Point'']]
 
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|1.5E-9 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
 
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|-
 
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|[[Part:BBa_F2620:Response time|'''Response time:''']]
 
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|<1 min
 
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|-
 
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|rowspan="2"|[[Part:BBa_F2620:Specificity|'''Input compatibility''']]
 
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|''Strong response to''
 
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|[[3OC6HSL|3OC<sub>6</sub>HSL]], C<sub>6</sub>HSL , C<sub>7</sub>HSL, 3OC<sub>8</sub>HSL, C<sub>8</sub>HSL
 
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|-
 
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|''Weak response to''
 
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|C<sub>4</sub>HSL, C<sub>10</sub>HSL, C<sub>12</sub>HSL
 
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|-
 
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|rowspan="2"|[[Part:BBa_F2620:Stability|'''Stability''']]
 
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|[[Genetic Stability|''Genetic Stability'']]<br>(Low/High Input)
 
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|>92/>56 generations
 
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|-
 
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|[[Performance Stability|''Performance Stability'']]<br>(Low/High Input)
 
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|>92/>56 generations
 
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|-
 
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|rowspan="4"|Demand
 
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|rowspan="1"|Internal Demand<br>(Low/High Input)
 
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|Not measured
 
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|-
 
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|rowspan="2"|[[Transcription Demand|''Transcriptional output demand:'']]<br>(Low/High Input)<br>Nt = length of downstream transcript in nucleotides
 
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|(0/6xNt) nucleotides cell<sup>-1</sup> s<sup>-1</sup>
 
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|-
 
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|(0/1.5E-1xNt) RNAP cell<sup>-1</sup>
 
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|-
 
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|[[Growth Rate|''Growth Rate'']]<br>(Low/High Input)
 
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|54/59 min Doubling time
 
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|}
 
 
</center>
 
</center>
 
<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
 
<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Revision as of 17:37, 26 October 2010

Input: L-Arabinose
Output: Light

pBad/araC
I0500		Luciferase/LRE
K325210
 Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        Add Data       


Description
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. D-Luciferin has to be added to obtain light output.

THIS PAGE IS CURRENTLY BEING UPDATED.

Pictures

Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)

</center> 1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3


References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17.

[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.

[http://www.ncbi.nlm.nih.gov/pubmed/11457857 [3]:] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39), 36508-36513.