Difference between revisions of "Part:BBa K364320:Experience"
Line 17: Line 17:
 
[[Image:nhr8pcdna.gif|800px|thumb|center|Picture of gel electrophoresis: Gal4-NHR8 in pCDNA3.1 resulting an insert of 953 bp.]]
 
[[Image:nhr8pcdna.gif|800px|thumb|center|Picture of gel electrophoresis: Gal4-NHR8 in pCDNA3.1 resulting an insert of 953 bp.]]
  
−
[[Image:Team Debrecen WB pCDNA-Gal4-NHR8.jpg|800px|thumb|center|]]
+
[[Image:Team Debrecen WB pCDNA-Gal4-NHR8.jpg|800px|thumb|center|Picture of Western blot: Gal4-NHR8 composite construct is highly expressed after transferring it into pCDNA3.1]]
 +
 
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K364320 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K364320 StartReviews</partinfo>

Revision as of 17:34, 26 October 2010


Physiological effects of NHR-8

NHR-8 is required for gut function and may respond to the food signal.

The nhr-8 gene mutants have abnormally low resistance to the toxins colchicine and chloroquine.This resembles the regulation of protective metabolic enzymes by xenobiotic-sensing nuclear receptors.

NHR-8 may thus mediate a subset of the resistance traits promoted by PGP-3.

Possible applications of GAL4-NHR-8

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364320

Picture of gel electrophoresis: Gal4-NHR8 in pCDNA3.1 resulting an insert of 953 bp.
Picture of Western blot: Gal4-NHR8 composite construct is highly expressed after transferring it into pCDNA3.1

User Reviews

UNIQ43af982bf8fe4763-partinfo-00000000-QINU UNIQ43af982bf8fe4763-partinfo-00000001-QINU