Difference between revisions of "Part:BBa K343006:Design"
CKurtzhals (Talk | contribs) (→Design Notes) |
CKurtzhals (Talk | contribs) (→Design Notes) |
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1. ''Source and function'' | 1. ''Source and function'' | ||
| − | The gene was cloned from ''Drosophila melanogaster'' cDNA aquired from [https://dgrc.cgb.indiana.edu/ Drosophila Genomic Resource Center]. The normal function of the gene is to create beta-caroten 15'15-monooxygenase as outlined above. The function of this gene has been characterized in the literature. | + | The gene was cloned from ''Drosophila melanogaster'' cDNA aquired from [https://dgrc.cgb.indiana.edu/ Drosophila Genomic Resource Center]. The normal function of the gene is to create beta-caroten 15'15-monooxygenase as outlined above. The function of this gene has been characterized in the literature.<br> |
2. ''Modifications before assembly'' | 2. ''Modifications before assembly'' | ||
| − | Appart from addition of BioBrick prefix/suffix no changes were made to the DNA before inserting it into ''E. coli''. | + | Appart from addition of BioBrick prefix/suffix no changes were made to the DNA before inserting it into ''E. coli''.<br> |
| − | + | ||
3. ''Vector'' | 3. ''Vector'' | ||
| − | The gene was inserted into a pSB1C3 backbone. | + | The gene was inserted into a pSB1C3 backbone.<br> |
| − | + | ||
6. ''Safety considderations'' | 6. ''Safety considderations'' | ||
Revision as of 17:27, 26 October 2010
Expresses B-carotene monooxygenase on a constitutive promotor
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 765
Illegal BamHI site found at 500
Illegal BamHI site found at 1757 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1361
Illegal BsaI site found at 1809
Design Notes
1. Source and function
The gene was cloned from Drosophila melanogaster cDNA aquired from Drosophila Genomic Resource Center. The normal function of the gene is to create beta-caroten 15'15-monooxygenase as outlined above. The function of this gene has been characterized in the literature.
2. Modifications before assembly
Appart from addition of BioBrick prefix/suffix no changes were made to the DNA before inserting it into E. coli.
3. Vector
The gene was inserted into a pSB1C3 backbone.
6. Safety considderations
We considered the gene, the strains of E. coli and plasmids used as safe.
Source
The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.