Difference between revisions of "Part:BBa K374008:Experience"
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The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it. The plasmid was transformed into ''E.coli'' (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. | The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it. The plasmid was transformed into ''E.coli'' (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. | ||
| + | From this construct no GFP expression is expected as repression of the pR promoter is tight. As a reference the repressor was removed from the construct. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:10, 26 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K374008
Biolector experiment
The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it. The plasmid was transformed into E.coli (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. From this construct no GFP expression is expected as repression of the pR promoter is tight. As a reference the repressor was removed from the construct.
User Reviews
UNIQ1c49ba4d6e54ce4b-partinfo-00000000-QINU UNIQ1c49ba4d6e54ce4b-partinfo-00000001-QINU