Difference between revisions of "Part:BBa K305007:Design"
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===Design Notes===
 
===Design Notes===
−
The sequence was retrieved from genomic ''Bacillus subtilis'' DNA using the following primers:
+
The sequence was retrieved from genomic ''Bacillus subtilis'' DNA using PCR with the following primers:
  
 
Srfa-short-for: ATCAGAATTCGCGGCCGCTTCTAGAGGATTGAACGCAGCAGTTTG
 
Srfa-short-for: ATCAGAATTCGCGGCCGCTTCTAGAGGATTGAACGCAGCAGTTTG

Revision as of 16:22, 26 October 2010

srfA promoter, short variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was retrieved from genomic Bacillus subtilis DNA using PCR with the following primers:

Srfa-short-for: ATCAGAATTCGCGGCCGCTTCTAGAGGATTGAACGCAGCAGTTTG

Srfa-short-rev: ACTACTGCAGCGGCCGCTACTAGTACCCACCCTAATAAGAAACC


Source

none

References