Difference between revisions of "Part:BBa K404206"

Line 16: Line 16:
 
   <th><b>Activity</b></th>
 
   <th><b>Activity</b></th>
 
   <th><b>Temp.</b></th>
 
   <th><b>Temp.</b></th>
</tr>
 
<tr>
 
  <td>upstream 453</td>
 
  <td>[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]</td>
 
  <td>[[image:Freiburg10_recognition site SspI-HF.Gif]]</td>
 
  <td>25;100;0;100</td>
 
<td>37°C</td>
 
 
  </tr>
 
  </tr>
 
<tr>
 
<tr>
   <td>downstream 453</td>
+
   <td>upstream 587</td>
   <td>[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]</td>
+
   <td>[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]</td>
   <td>[[image:Freiburg10_recognition site SalI-HF.Gif]]</td>
+
   <td>[[image:Freiburg10_recognition site BamHI-HF.Gif]]</td>
   <td>10;100;100;100</td>
+
   <td>100;50;10;100</td>
 
   <td>37°C</td>
 
   <td>37°C</td>
 
+
</tr>
 +
<tr>
 +
  <td>downstream 587</td>
 +
  <td>[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]</td>
 +
  <td>[[image:Freiburg10_recognition site PvuII-HF.Gif]]</td>
 +
  <td>0;25;0;100</td>
 +
  <td>37°C
 +
</tr>
 
<tr>
 
<tr>
 
</table>
 
</table>
 +
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 15:52, 26 October 2010

ViralBrick-587-His-Tag

Freiburg10 ViralBrick-logo-587-His.png

Usage and Biology

Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 587 [http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF] Freiburg10 recognition site BamHI-HF.Gif 100;50;10;100 37°C
downstream 587 [http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF] Freiburg10 recognition site PvuII-HF.Gif 0;25;0;100 37°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]