Difference between revisions of "Part:BBa K404205"

Revision as of 15:51, 26 October 2010

ViralBrick-587-BAP
The Biotinylation Acceptor peptide motif, ready for insertion into the 587 loop

</html>

Restriction site	Enzyme	Recognition sequence	Activity	Temp.
upstream 587	[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]		100;50;10;100	37°C
downstream 587	[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]		0;25;0;100	37°C

Sequence and Features

Assembly Compatibility:

@@ Line 10: / Line 10: @@
 ===Restriction sites===
-<table border="1" style="float:right; width:480px; height:auto;>
+</html><table border="1" style="float:right; width:480px; height:auto;>
   <tr>
    <th><b>Restriction site</b></th>
@@ Line 17: / Line 17: @@
    <th><b>Activity</b></th>
    <th><b>Temp.</b></th>
- </tr>
- <tr>
-  <td>upstream 453</td>
-  <td>[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]</td>
-  <td>[[image:Freiburg10_recognition site SspI-HF.Gif]]</td>
-  <td>25;100;0;100</td>
- <td>37°C</td>
   </tr>
 <tr>
-   <td>downstream 453</td>
+   <td>upstream 587</td>
-   <td>[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]</td>
+   <td>[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]</td>
-   <td>[[image:Freiburg10_recognition site SalI-HF.Gif]]</td>
+   <td>[[image:Freiburg10_recognition site BamHI-HF.Gif]]</td>
-   <td>10;100;100;100</td>
+   <td>100;50;10;100</td>
    <td>37°C</td>
+ </tr>
+<tr>
+  <td>downstream 587</td>
+  <td>[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]</td>
+  <td>[[image:Freiburg10_recognition site PvuII-HF.Gif]]</td>
+  <td>0;25;0;100</td>
+  <td>37°C
+ </tr>
 <tr>
 </table>