Difference between revisions of "Part:BBa K404204"

Revision as of 15:45, 26 October 2010

ViralBrick-453-Z34C
The Z34C antibody binding motif, ready for insertion into the 453 loop

Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Restriction sites

Restriction site	Enzyme	Recognition sequence	Activity	Temp.
upstream 453	[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]		25;100;0;100	37°C
downstream 453	[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]		10;100;100;100	37°C

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 [[Image:Freiburg10_ViralBrick_motif_Z34C.png|thumb|center|480px]]
+===Restriction sites===
+<table border="1" style="float:right; width:480px; height:auto;>
+ <tr>
+  <th><b>Restriction site</b></th>
+  <th><b>Enzyme</b></th>
+  <th><b>Recognition sequence</b></th>
+  <th><b>Activity</b></th>
+  <th><b>Temp.</b></th>
+ </tr>
+ <tr>
+  <td>upstream 453</td>
+  <td>[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]</td>
+  <td>[[image:Freiburg10_recognition site SspI-HF.Gif]]</td>
+  <td>25;100;0;100</td>
+ <td>37°C</td>
+ </tr>
+<tr>
+  <td>downstream 453</td>
+  <td>[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]</td>
+  <td>[[image:Freiburg10_recognition site SalI-HF.Gif]]</td>
+  <td>10;100;100;100</td>
+  <td>37°C</td>
+<tr>
+</table>
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 <span class='h3bb'>Sequence and Features</span>