Difference between revisions of "Part:BBa K389017"
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<partinfo>BBa_K389017 short</partinfo>
 
<partinfo>BBa_K389017 short</partinfo>
  
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This BioBrick is a VirA/G signaling system from ''Agrobacterium tumefaciens'' without read-out. It contains an unmutated ''virA'' gene and a ''virG'' gene that works without ''rpoA'' from ''A. tumefaciens'' and a ''vir'' promoter.  
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This BioBrick is a VirA/G signaling system from ''Agrobacterium tumefaciens'' without read-out. It contains an unmutated ''virA'' gene and a ''virG'' gene that works without ''rpoA'' from ''A. tumefaciens'' and a ''vir'' promoter. In the presence of the inducer acetosyringone VirA phosphorylates VirG and this activates the ''vir'' promoter.  
  
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Input: acetosyringone
 
  
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Output: PoPS
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===Parameters===
  
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Characterizations of this part was made with <partinfo>K389015</partinfo> and <partinfo>K389016</partinfo>. Look at the description of these parts to get more detailed information about the VirA/G signaling system.  
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Characterizations of this part were made with <partinfo>K389015</partinfo> and <partinfo>K389016</partinfo>. Look at the description of these parts to get more detailed information about the VirA/G signaling system. In the following table, some important parameters of the VirA/G signaling system are summarized:
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<center>
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{|cellpadding="10" style="border-collapse: collapse; border-width: 1px; border-style: solid; border-color: #000"
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|-
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!style="border-style: solid; border-width: 1px"| Parameter
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!style="border-style: solid; border-width: 1px"| Result
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|-
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|style="border-style: solid; border-width: 1px"| Input
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|style="border-style: solid; border-width: 1px"| Acetosyringone
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|-
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|style="border-style: solid; border-width: 1px"| Output
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|style="border-style: solid; border-width: 1px"| PoPS
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|-
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|style="border-style: solid; border-width: 1px"| Switch point
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|style="border-style: solid; border-width: 1px"| ~ 30 µM acetosyringone
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|-
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|style="border-style: solid; border-width: 1px"| Fully induced
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|style="border-style: solid; border-width: 1px"| > 150 µM acetosyringone
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|-
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|style="border-style: solid; border-width: 1px"| Increased expression when induced
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|style="border-style: solid; border-width: 1px"| > 2.2 fold (compared to uninduced system)
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|-
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|style="border-style: solid; border-width: 1px"| Hill coefficient
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|style="border-style: solid; border-width: 1px"| positive cooperation (> 1)
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|}
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</center>
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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Revision as of 15:14, 26 October 2010

VirA/G signaling system without read-out

This BioBrick is a VirA/G signaling system from Agrobacterium tumefaciens without read-out. It contains an unmutated virA gene and a virG gene that works without rpoA from A. tumefaciens and a vir promoter. In the presence of the inducer acetosyringone VirA phosphorylates VirG and this activates the vir promoter.


Parameters

Characterizations of this part were made with BBa_K389015 and BBa_K389016. Look at the description of these parts to get more detailed information about the VirA/G signaling system. In the following table, some important parameters of the VirA/G signaling system are summarized:

Parameter Result
Input Acetosyringone
Output PoPS
Switch point ~ 30 µM acetosyringone
Fully induced > 150 µM acetosyringone
Increased expression when induced > 2.2 fold (compared to uninduced system)
Hill coefficient positive cooperation (> 1)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768