Difference between revisions of "Part:BBa K305011"
| Line 2: | Line 2: | ||
<partinfo>BBa_K305011 short</partinfo> | <partinfo>BBa_K305011 short</partinfo> | ||
| − | Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis <cite>Bongers</cite>, this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction. | + | Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis <cite>Bongers</cite>, this plasmid was constructed to express proteins present in RCF 10 BioBricks, composed of an RBS followed by a coding sequence, upon subtilin induction. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 14:12, 26 October 2010
Subtilin inducible expression plasmid for Bacillus subtilis
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis Bongers, this plasmid was constructed to express proteins present in RCF 10 BioBricks, composed of an RBS followed by a coding sequence, upon subtilin induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3141
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3147 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3141
Illegal BglII site found at 3060
Illegal XhoI site found at 287 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3141
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3141
Plasmid lacks a suffix.
Illegal XbaI site found at 3156
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 324
References
<biblio>
- Bongers pmid=16332878
</biblio>