Difference between revisions of "Part:BBa K404251"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404251 short</partinfo>
 
<partinfo>BBa_K404251 short</partinfo>
−
<br><b>The Biotinylation Acceptor peptide motif, ready for insertion into the 453 loop</b>
+
<br><b>The Biotinylation Acceptor peptide motif, including a knockout of the natural HSPG tropism, inserted into the 587 loop of [[Part:BBa_K404007|pCMV_[AAV2]-VP123]]</b>
  
 
[[Image:Freiburg10_pCMV_VP123 587KO-BAP.png|thumb|center|480px]]<br>
 
[[Image:Freiburg10_pCMV_VP123 587KO-BAP.png|thumb|center|480px]]<br>

Revision as of 12:33, 26 October 2010

pCMV_[AAV2]-VP123ex (ViralBrick-587KO-BAP)
The Biotinylation Acceptor peptide motif, including a knockout of the natural HSPG tropism, inserted into the 587 loop of pCMV_[AAV2]-VP123

Freiburg10 pCMV VP123 587KO-BAP.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2396
    Illegal XhoI site found at 698
    Illegal XhoI site found at 884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2982
    Illegal SapI site found at 1833