Difference between revisions of "Part:BBa K404167"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404167 short</partinfo>
 
<partinfo>BBa_K404167 short</partinfo>
−
<br><b>The Biotinylation Acceptor peptide motif, ready for insertion into the 453 loop</b>
+
<br><b><br><b>Construct containing the mVenus N-terminal fusion to VP2, a NLS & Vp1 insertsion and the Biotinylation Acceptor peptide motif in the 587 loop</b></b>
  
  

Revision as of 11:49, 26 October 2010

pCMV_VP1up_NLS_mVenus_VP23(ViralBrick-587-KO-BAP)

<b>Construct containing the mVenus N-terminal fusion to VP2, a NLS & Vp1 insertsion and the Biotinylation Acceptor peptide motif in the 587 loop</b>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3149
    Illegal XhoI site found at 698
    Illegal XhoI site found at 884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3735
    Illegal SapI site found at 2586