Difference between revisions of "Part:BBa K338003"

 
 
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<partinfo>BBa_K338003 short</partinfo>
 
<partinfo>BBa_K338003 short</partinfo>
  
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This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains only ''phaA'': <partinfo>BBa_K156012</partinfo>. It was designed to be ligated upstream of part [[Team:Caltech/BBa_K338004|BBa_K338004]].
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This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains only ''phaA'': <partinfo>BBa_K156012</partinfo>. It was designed to be ligated upstream of part <partinfo>BBa_K338004</partinfo>.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:15, 26 October 2010

PHA Synthase Composite, Part 1/2

This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains only phaA: BBa_K156012. It was designed to be ligated upstream of part BBa_K338004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 338
    Illegal AgeI site found at 701
  • 1000
    COMPATIBLE WITH RFC[1000]