Difference between revisions of "Part:BBa K346030"

Latest revision as of 08:18, 26 October 2010

T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of lead binding peptide)

This part is composed of a T7promoter, a rbs and the periplasm display protein Dsba-MBP, which binding the lead of the enviroment. DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically. The construction of Dsba-MBP(for lead) is similar to that part for mercury binding.more details[[1]]

Here is the results of our experiments.

Figure 1: The expression of DsbA+MBP. This MBP is engineered to bind lead more efficiently. We use this part to capture lead in the periplasm.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 520
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 520
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 308
Illegal XhoI site found at 1005
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 520
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 520
Illegal AgeI site found at 140
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 This part is composed of a T7promoter, a rbs and the periplasm display protein Dsba-MBP, which binding the lead of the enviroment.
-DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .
+DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically. The construction of Dsba-MBP(for lead) is similar to that part for mercury binding.more details[[https://parts.igem.org/Part:BBa_K346033]]
+Here is the results of our experiments.
 [[Image:pb dsab.jpg]]