Difference between revisions of "Part:BBa K346029"

Latest revision as of 07:49, 26 October 2010

T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of lead binding peptide)

This part is the combination of T7promoter,rbs and the lpp-ompa-mbp,which expresses the surface-display protein to bind the lead in the environment.

Description

Just as what is designed in the mercury absorption device, Lpp-OmpA-mbp(lead) is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP), which functions as the surface display part to bind lead in the water. The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of OmpA serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.The predicted structure is shown below.

Figure 1: The predicted structure of lpp-OmpA-MBP. This part is designed to bind heavy metal on the surface via MBP, which is said to be the most effective and efficient.

Figure 2: The expression of lpp-OmpA-MBP. We can see a clear band about 27KD both in SDS-PAGE and Western Blotting result.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 '''Description'''
-Just as what is designed in the mercury absorption device, Lpp-OmpA-mbp(lead) is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP), which plays as the surface display part to bind lead in the water. The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.The predicted structure is shown below.
+Just as what is designed in the mercury absorption device, Lpp-OmpA-mbp(lead) is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP), which functions as the surface display part to bind lead in the water. The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of OmpA serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.The predicted structure is shown below.
 [[Image:pblom.jpg]]
+Figure 1: The predicted structure of lpp-OmpA-MBP. This part is designed to bind heavy metal on the surface via MBP, which is said to be the most effective and efficient.
+[[Image:xpe adf.jpg]]
+Figure 2: The expression of lpp-OmpA-MBP. We can see a clear band about 27KD both in SDS-PAGE and Western Blotting result.
 <!-- Add more about the biology of this part here