Difference between revisions of "Part:BBa K238008"
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<partinfo>BBa_K238008 short</partinfo> | <partinfo>BBa_K238008 short</partinfo> | ||
| − | This is VirA, a histidine kinase sensor originally found in Agrobacterium tumefaciens, Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly. | + | This is VirA, a histidine kinase sensor originally found in ''Agrobacterium tumefaciens'', Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly. |
VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA. | VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA. | ||
Latest revision as of 06:41, 26 October 2010
VirA
This is VirA, a histidine kinase sensor originally found in Agrobacterium tumefaciens, Ti plasmid. It is part of the two-component VirA/VirG signal transduction system. This system is able to sense phenolic compounds and regulate gene transcription correspondingly.
VirA has illegal PstI restriction sites in its sequence. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 502
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 666
Illegal BglII site found at 1559 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1405
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1177
Illegal BsaI site found at 1969
Illegal SapI site found at 1651
Illegal SapI.rc site found at 880