Difference between revisions of "Part:BBa K346032"
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Figure 1: The structure of MBP we designed. The ball indicate the binding heavy metal. Noting that this MBP is constructed by duplicating two helix which is the metal-binding domain of MerR and connect them using a linker. | Figure 1: The structure of MBP we designed. The ball indicate the binding heavy metal. Noting that this MBP is constructed by duplicating two helix which is the metal-binding domain of MerR and connect them using a linker. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 06:01, 26 October 2010
T7promoter(I719005)_RBS(B0034)_MBP(mercury metal binding peptide)
This part is a combination of T7promoter(I719005),RBS(B0034) and MBP(mercury metal binding peptide). MBP is short for metal binding peptide. It is designed by our team by duplicating MerR's metal binding sites and connect them using a link.
By constructing this part, we are able to express MBP in the cytosol to bind heavy metal within the cell.
Figure 1: The structure of MBP we designed. The ball indicate the binding heavy metal. Noting that this MBP is constructed by duplicating two helix which is the metal-binding domain of MerR and connect them using a linker.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 215
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]