Difference between revisions of "Part:BBa K346033"
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<partinfo>BBa_K346033 short</partinfo> | <partinfo>BBa_K346033 short</partinfo> | ||
This part is a combination of T7promoter(BBa_I719005),RBS(B0034)and DsbA-MBP(periplasm display of mercury binding peptide) | This part is a combination of T7promoter(BBa_I719005),RBS(B0034)and DsbA-MBP(periplasm display of mercury binding peptide) | ||
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| + | '''Description''' | ||
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| + | DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically . | ||
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| + | [[Image:Dsba-MBP.jpg]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 04:32, 26 October 2010
T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of mercury binding peptide)
This part is a combination of T7promoter(BBa_I719005),RBS(B0034)and DsbA-MBP(periplasm display of mercury binding peptide)
Description
DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 529
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 529
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 317
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 529
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 529
Illegal AgeI site found at 149 - 1000COMPATIBLE WITH RFC[1000]