Difference between revisions of "Part:BBa K346029"

Revision as of 04:10, 26 October 2010

T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of lead binding peptide)

This part is the combination of T7promoter,rbs and the lpp-ompa-mbp,which expresses the surface-display protein to bind the lead in the environment.

Description

Just as what is designed in the mercury absorption device, Lpp-OmpA-mbp(lead) is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP), which plays as the surface display part to bind lead in the water. The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.The predicted structure is shown below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K346029 short</partinfo>
 This part is the combination of T7promoter,rbs and the lpp-ompa-mbp,which expresses the surface-display protein to bind the lead in the environment.
+'''Description'''
+Just as what is designed in the mercury absorption device, Lpp-OmpA-mbp(lead) is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP), which plays as the surface display part to bind lead in the water. The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.The predicted structure is shown below.
+[[Image:pblom.jpg]]
 <!-- Add more about the biology of this part here