Difference between revisions of "Part:BBa K346085:Design"
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<partinfo>BBa_K346085 short</partinfo> | <partinfo>BBa_K346085 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Just ligate the promoter, RBS and T7polymerase together by subclone to get the generator. | |
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===Source=== | ===Source=== | ||
| − | Ptet | + | It is necessary to point that Ptet is a constitutive promoter only in the trans5alpha strain. |
===References=== | ===References=== | ||
Latest revision as of 03:48, 26 October 2010
Ptet-RBS-T7polymerase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Just ligate the promoter, RBS and T7polymerase together by subclone to get the generator.
Source
It is necessary to point that Ptet is a constitutive promoter only in the trans5alpha strain.