Difference between revisions of "Part:BBa K415000:Design"
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<partinfo>BBa_K415000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K415000 SequenceAndFeatures</partinfo>
  
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BBa_K415000 consists of a Plux_cI promoter expressing mCherry. The part was constructed via the insertion of BBa_J06702 into a BBa_R0065 backbone(pSB1A2). The following gel presents confirmation of said construction:
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BBa_K415000 consists of a Plux_cI promoter expressing mCherry. The part was constructed via the insertion of BBa_J06702 into a BBa_R0065 backbone(pSB1A2). The following gel, run against Hyperladder 1 presents confirmation of said construction:
  
  

Revision as of 03:47, 26 October 2010

pLux/cI-OR : RBS-mCherry : Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

BBa_K415000 consists of a Plux_cI promoter expressing mCherry. The part was constructed via the insertion of BBa_J06702 into a BBa_R0065 backbone(pSB1A2). The following gel, run against Hyperladder 1 presents confirmation of said construction:


K415000 gel.jpg


The following is a plasmid map of the part in the pSB1A2 backbone:


K415000 geneious.jpg


The fluorescence of mCherry was then quantified using FACs(Fluorescence-activated cell sorting) running two samples: -AHL and +AHL. The addition of AHL tests whether or not the circuit will respond without LuxR.


K415000.jpg


It is clear that the circuit does not respond to the addition of AHL. The following are FACs sheets:

-AHL

K415000Basal.jpg


+AHL

K415000+AHL facs.jpg



Source

https://parts.igem.org/wiki/index.php?title=Part:BBa_R0065

https://parts.igem.org/wiki/index.php?title=Part:BBa_J06702

References