Difference between revisions of "Part:BBa K346036"

Revision as of 03:37, 26 October 2010

PT7-RBS-lpp-ompa-mbp-terminator

PT7-RBS-Lpp-OmpA-MBP-Terminator

Description

It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts.

Lpp-OmpA-mbp is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP). The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 660
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K346036 short</partinfo>
-Ptet-T7polymerase+Pb(II) Bioabsorbption Device
+PT7-RBS-Lpp-OmpA-MBP-Terminator
+== Description ==
+It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts.
+Lpp-OmpA-mbp is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP). The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.
+        [[Image:Lpp-OmpA-MBP.jpg]]