Difference between revisions of "Part:BBa K346035"

Latest revision as of 03:31, 26 October 2010

Ptet-T7polymerase+Hg(II) Bioabsorption Device

This part is made up of a Ptet-T7polymerase and the Hg(II) Bioabsorption Device. The Ptet-rbs-T7polymerase can constotuitively express in the DH5alpha strain, which can drive the Hg(II) bioabsorption device. the Bioabsorption Device is combined with the Dsba-MBP, MBP and lpp-OmpA-MBP. MBP here refers to the mercury metal binding peptide.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 529
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 529
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 317
Illegal BamHI site found at 1148
Illegal BamHI site found at 2111
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 529
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 529
Illegal AgeI site found at 149
1000
COMPATIBLE WITH RFC[1000]

Revision as of 03:30, 26 October 2010 (view source) JjunyiJiao (Talk \| contribs) ← Older edit		Latest revision as of 03:31, 26 October 2010 (view source) JjunyiJiao (Talk \| contribs)
Line 4:		Line 4:
	This part is made up of a Ptet-T7polymerase and the Hg(II) Bioabsorption Device. The Ptet-rbs-T7polymerase can constotuitively express in the DH5alpha strain, which can drive the Hg(II) bioabsorption device. the Bioabsorption Device is combined with the Dsba-MBP, MBP and lpp-OmpA-MBP. MBP here refers to the mercury metal binding peptide.		This part is made up of a Ptet-T7polymerase and the Hg(II) Bioabsorption Device. The Ptet-rbs-T7polymerase can constotuitively express in the DH5alpha strain, which can drive the Hg(II) bioabsorption device. the Bioabsorption Device is combined with the Dsba-MBP, MBP and lpp-OmpA-MBP. MBP here refers to the mercury metal binding peptide.

−	[[t7-dml.jpg]]	+	[[Image:t7-dml.jpg]]