Difference between revisions of "Part:BBa K349002"

Revision as of 02:53, 26 October 2010

RFP-BA-BsaI (for construction of BA BioBytes 2.0)

Base Plasmid v.2 for the construction of new BioBytes-v2. The RFP cassette can be removed using the BsaI enzyme and a new BA linker style part may be inserted into the plasmid allowing for testing of the part with the BioBytes Assembly System-v2.

Usage and Biology

This plasmid is used to create BA linker style parts for the BioByte Assembly Method v.2. The RFP coding cassette provides a selection method for your parts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 792
Illegal AgeI site found at 904
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1086

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K349002 short</partinfo>
-Test plasmid for the construction of new Bytes-v2.  The RFP cassette can be removed using the BsaI enzyme and a new ORF or cassette may be inserted into the plasmid allowing for testing of the part with the BioBytes Assembly System-v2.
+Base Plasmid v.2 for the construction of new BioBytes-v2.  The RFP cassette can be removed using the BsaI enzyme and a new BA linker style part may be inserted into the plasmid allowing for testing of the part with the BioBytes Assembly System-v2.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+This plasmid is used to create BA linker style parts for the BioByte Assembly Method v.2.  The RFP coding cassette provides a selection method for your parts.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K349002 SequenceAndFeatures</partinfo>