Difference between revisions of "Part:BBa K349001"

Revision as of 02:41, 26 October 2010

RFP-AB-BsaI (for construction of AB BioBytes 2.0)

Base plasmid v.2 for the construction of BioBytes-v2. The RFP cassette can be removed using the BsaI enzyme and a new AB ORF style part may be inserted into the plasmid for amplification.

Usage and Biology

This plasmid is used to create AB ORF style parts for the BioByte Assembly Method v.2.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 792
Illegal AgeI site found at 904
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1086

Revision as of 02:41, 26 October 2010 (view source) KarinaA (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 02:41, 26 October 2010 (view source) KarinaA (Talk \| contribs) Newer edit →
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−	This plasmid is used to create AB ORF style parts for the BioByte Assembly Method v.2.	+	This plasmid is used to create AB ORF style parts for the BioByte Assembly Method v.2.