Difference between revisions of "Part:BBa K349001"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K349001 short</partinfo>
 
<partinfo>BBa_K349001 short</partinfo>
  
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Test plasmid for the construction of new Bytes-v2.  The RFP cassette can be removed using the BsaI enzyme and a new ORF or cassette may be inserted into the plasmid allowing for testing of the part with the BioBytes Assembly System-v2.
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Base plasmid v.2 for the construction of BioBytes-v2.  The RFP cassette can be removed using the BsaI enzyme and a new AB ORF style part may be inserted into the plasmid for amplification.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This plasmid is used to create AB ORF style parts for the BioByte Assembly Method v.2.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K349001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K349001 SequenceAndFeatures</partinfo>

Revision as of 02:40, 26 October 2010

RFP-AB-BsaI (for construction of AB BioBytes 2.0)

Base plasmid v.2 for the construction of BioBytes-v2. The RFP cassette can be removed using the BsaI enzyme and a new AB ORF style part may be inserted into the plasmid for amplification.


Usage and Biology

This plasmid is used to create AB ORF style parts for the BioByte Assembly Method v.2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1086