Difference between revisions of "Part:BBa K302018:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K302018 short</partinfo>
 
<partinfo>BBa_K302018 short</partinfo>
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===Source===
 
===Source===
  
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Gene Bank
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''Bacillus subtilis'' ATCC 6633 genome.
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===References===
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Latest revision as of 01:56, 26 October 2010

Subtilin immunity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 837
    Illegal BglII site found at 1585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1132
    Illegal SapI site found at 1412
    Illegal SapI.rc site found at 2135


Design Notes

SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA.


Source

Bacillus subtilis ATCC 6633 genome.