Difference between revisions of "Part:BBa K302035:Design"
Swoodhouse (Talk | contribs) (→Design Notes) |
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*Sequence selected based on the work of Pellegrini at al. and Shimotsu and Henner in the papers below. | *Sequence selected based on the work of Pellegrini at al. and Shimotsu and Henner in the papers below. | ||
*Organisms with this device integrated must be grown on 10% sucrose media to be viable. | *Organisms with this device integrated must be grown on 10% sucrose media to be viable. | ||
| − | *Should be integrated at the ''sacB'' locus to prevent native Levansucrose glue production (to use in conjunction with Levansucrose glue, use part [Part:BBa_K302030 BBa_K302030]). | + | *Should be integrated at the ''sacB'' locus to prevent native Levansucrose glue production (to use in conjunction with Levansucrose glue, use part [[Part:BBa_K302030|BBa_K302030]]). |
===Source=== | ===Source=== | ||
Latest revision as of 01:15, 26 October 2010
Sucrose-limitation induced kill switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 363
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Sequence selected based on the work of Pellegrini at al. and Shimotsu and Henner in the papers below.
- Organisms with this device integrated must be grown on 10% sucrose media to be viable.
- Should be integrated at the sacB locus to prevent native Levansucrose glue production (to use in conjunction with Levansucrose glue, use part BBa_K302030).
Source
Bacillus Subtilis 168 strain
References
Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol 56: 1139–1148.
Shimotsu, H., and Henner, D. J. (1986) J. Bacteriol. 168, 380–388