Difference between revisions of "Part:BBa K349008"

 
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<partinfo>BBa_K349008 short</partinfo>
 
<partinfo>BBa_K349008 short</partinfo>
  
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BioByte Assembly Method-v2 testing plasmid containing the kanamycin resistance gene.  Digestion with BsaI allows for the addition of any other ORF or part to the plasmid.
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BioByte Assembly Method-v2 base plasmid v.1 containing the kanamycin resistance gene.  Digestion with BsaI allows for the addition of any other BA linker style part to the plasmid.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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This plasmid is used to create BA linker style parts for the BioByte Assembly Method v.2. 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K349008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K349008 SequenceAndFeatures</partinfo>

Revision as of 00:53, 26 October 2010

KanR-BA-BsaI (for construction of BA BioBytes 2.0)

BioByte Assembly Method-v2 base plasmid v.1 containing the kanamycin resistance gene. Digestion with BsaI allows for the addition of any other BA linker style part to the plasmid.


Usage and Biology

This plasmid is used to create BA linker style parts for the BioByte Assembly Method v.2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 984