Difference between revisions of "Part:BBa K302035"
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| − | Encodes a stable non-specific ribonuclease toxin (''mazF'') and its inhibitory antitoxin (''mazE'') in ''Bacillus | + | Encodes a stable non-specific ribonuclease toxin (''mazF'') and its inhibitory antitoxin (''mazE'') in ''Bacillus subtilis''. Contains Sucrose sensitive inducer that will only allow coding sequence translation in the presence of sucrose. These genes are used in ''Bacillus subtilis'' to provide a toxin-antitoxin kill switch in various stressful conditions. When translation of both genes is turned off by sucrose limitation ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''mazF'', killing the cell. |
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Revision as of 00:48, 26 October 2010
Sucrose-limitation induced kill switch
Encodes a stable non-specific ribonuclease toxin (mazF) and its inhibitory antitoxin (mazE) in Bacillus subtilis. Contains Sucrose sensitive inducer that will only allow coding sequence translation in the presence of sucrose. These genes are used in Bacillus subtilis to provide a toxin-antitoxin kill switch in various stressful conditions. When translation of both genes is turned off by sucrose limitation mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 363
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]